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MICs of all tested antibiotics for each Borrelia strain
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MICs of all tested antibiotics for each Borrelia strain

Journal: Antimicrobial Agents and Chemotherapy

Article Title: In Vitro Antimicrobial Susceptibility of Clinical Isolates of Borrelia miyamotoi

doi: 10.1128/AAC.00419-18

Figure Lengend Snippet: MICs of all tested antibiotics for each Borrelia strain

Article Snippet: Interestingly, the amoxicillin MIC m values of both B. miyamotoi tick isolates, all B. miyamotoi clinical isolates, and the B. hermsii control strain were significantly higher than the clinical breakpoint for resistance (≤4 mg/liter, according to the Clinical and Laboratory Standards Institute [CLSI]) ( 15 , 16 ).

Techniques:

Decrease in absorbance after incubation with amoxicillin. Absorbance was measured at 562 (corrected for absorbance at 630 nm), and the decrease in absorbance was measured after 72 h of incubation with amoxicillin and compared with the decrease in absorbance of the positive-control sample ([Et0 − Et72] of <25% of [EPOS,t0 − EPOS,t72]). Shown are representative results of two individual experiments with five representative Borrelia strains, i.e., PKo (B. afzelii), N40 (B. burgdorferi sensu stricto), HS1 (B. hermsii from the United States), and Izh-14 and Yek-1 (B. miyamotoi clinical isolates). Symbols and error bars represent the mean ± standard error of the mean of quadruplicate results. If no error bars are visible, then the error bars were smaller than the symbol.

Journal: Antimicrobial Agents and Chemotherapy

Article Title: In Vitro Antimicrobial Susceptibility of Clinical Isolates of Borrelia miyamotoi

doi: 10.1128/AAC.00419-18

Figure Lengend Snippet: Decrease in absorbance after incubation with amoxicillin. Absorbance was measured at 562 (corrected for absorbance at 630 nm), and the decrease in absorbance was measured after 72 h of incubation with amoxicillin and compared with the decrease in absorbance of the positive-control sample ([Et0 − Et72] of <25% of [EPOS,t0 − EPOS,t72]). Shown are representative results of two individual experiments with five representative Borrelia strains, i.e., PKo (B. afzelii), N40 (B. burgdorferi sensu stricto), HS1 (B. hermsii from the United States), and Izh-14 and Yek-1 (B. miyamotoi clinical isolates). Symbols and error bars represent the mean ± standard error of the mean of quadruplicate results. If no error bars are visible, then the error bars were smaller than the symbol.

Article Snippet: Interestingly, the amoxicillin MIC m values of both B. miyamotoi tick isolates, all B. miyamotoi clinical isolates, and the B. hermsii control strain were significantly higher than the clinical breakpoint for resistance (≤4 mg/liter, according to the Clinical and Laboratory Standards Institute [CLSI]) ( 15 , 16 ).

Techniques: Incubation, Positive Control